Boletín semanal de ofertas de empleo publico
Institución:Institute of Biomedicine of Seville
Dr. Ezequiel Ruiz-Mateos is looking for candidates for a PhD student position at Institute of Biomedicine of Seville associated to "La Caixa, Fellowship Programme INPhiNIT". Applicants for any nationality are allowed always that they have not spent more than twelve months in Spain in the last three years. Applicants must not have started the PhD studies. Applicants with more than 2.5 mark or equivalent in the undergraduate studies are desirable. Please if you are interested send your CV to firstname.lastname@example.org before the 30th of January.
-Research Project / Research Group Description:
Disturbances in the innate arm of immunity have important deleterious effects in aging and HIV-infection. Both scenarios are characterized by a low grade chronic inflammation related with age-associated diseases. In HIV-infection, these diseases are termed non AIDS events (NAEs); the main cause of mortality in HIV-infected patients on suppressive antiretroviral therapy (cART) (currently, 80-90% of the patients). In my group, we have observed in these patients that parameters associated with monocyte activation and specifically with the phenotype and functionality of these cells are deeply deregulated. A growing body of evidence indicates that another innate immunity component playing a critical role in HIV pathogenesis, is the plasmacytoid dendritic cell (pDC).These cells are the main type I Interferon (IFN-I) producers. pDCs sense virus and bacteria through endosomal Toll like receptors (TLRs)-7 and -9. Therefore, pDCs produce IFN which confers to this cell a high antiviral
capacity, activate antiviral host restriction factors and at the same time matures, expressing co-stimulatory molecules and become antigen presenting cells (APCs) enabling the expansion of CD4+ and CD8+ T-cells. This makes pDCs an essential link between innate and adaptive immunity. All this exhaustive coordinated process is severely disturbed by HIV for its own benefit. Key feature of this deregulation is an aberrant IFN production which misbalances an "antigen presenting pDC phenotype" in favor of an "IFN producing pDC phenotype". The cellular basis of this process is not completely understood.
Hypothesis: Different characteristics of pDC phenotype and functions are associated with different disease outcomes in HIV infection. These special characteristics and functions of the pDC, linking innate and adaptive immunity, allow the spontaneous control of HIV. On the contrary, the deregulation of the pDC in typical progressors induces a permanent activation and hyperinflammation that eventually favor the appearance of NAEs.
Objective: To analyze differential phenotypic and functional mechanisms of pDCs associated with different disease outcomes in HIV infection.
Societal Impact: Dissecting the mechanisms of a potential preserved pDC function in EC compared to progressors will help to identify molecular targets.
-Job position description:
The PhD student will be responsible of all the experimental procedures associated with the present project. He/She will be supervised by the group leader and results will be presented in national and international meetings and published in peer reviewed international journals.
The study will be performed at the Immunovirology Laboratory, Biomedicine Institute of Seville (IBiS)/Virgen del Rocio University Hospital, Seville, Spain. The setting of the laboratory is ideal for the development of a translational research. On one hand, the Immunovirology Laboratory is part of the Clinical Unit of Infectious Diseases Department at Virgen del Rocio University Hospital. This is a reference Unit in the field of HIV research in Spain with a great number of surgeries specialized in HIV management. The HIV-infected patients' cohort of the Infectious Diseases Unit is one of the biggest in Spain with around 6,000 patients. The clinical staff of the Univt work together with the basic staff that translates the problems from the clinic to the bench, the achievement of the aim of the present proposal will allow reverting this knowledge for the benefit of the patients as a result of a high quality translational research. Samples from the patients and from all the pat
ients of the cohort are kept in the Immunovirology Laboratory Biobank since 1991, part of the Andalusian Health Service Biobank. In the Biobank; PBMCs, serum, plasma, DNA and tissue samples are kept in accurate conditions to be used in the experiments of the present proposal. On the other hand, the Biomedicine Institute of Seville (IBiS), offers a critical support for the proposal. IBiS is a multidisciplinary biomedical research centre located in the campus of Virgen del Rocio University Hospital, and through the European project ITRIBIS "Improving Translational Research Potential at the Institute of Biomedicine of Seville" IBiS is envisaged to become a reference of biomedical translational research applied to prevalent diseases in South Europe. IBIS is based on fundamental research at the molecular and cellular level with a view to promote the rapid transfer of knowledge to the clinical setting, at the same time improving the quality of clinical and epidemiological research
Información complementaria de la oferta: